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Journal: bioRxiv
Article Title: Use of Epivolve phage display to generate a monoclonal antibody with opsonic activity directed against a subdominant epitope on extracellular loop 4 of Treponema pallidum BamA (TP0326)
doi: 10.1101/2023.05.13.540667
Figure Lengend Snippet: A peptide incorporating a non-native amino acid at a desired site is used to pan a scFv phage library for peptide binders. Phages that bind the modified, but not the native, peptide undergo AXM mutagenesis to generate phages that recognize the native peptide +/-the modified peptide with high affinity.
Article Snippet: The template plasmid for all phage display libraries is a derivative of the phagemid pIT2 ( ) with a human
Techniques: Modification, Mutagenesis